The brain-specific gene for rat aldolase C possesses an unusual housekeeping-type promoter
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چکیده
منابع مشابه
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Human glycophorin C (GPC) is an integral membrane protein found in many cell types but which exhibits increased expression in red cells. We have investigated the in vitro binding of nuclear protein factors to the GPC gene 5'-flanking region which was transcriptionally active when transfected into erythroid and non-erythroid cells. We found that in addition to known ubiquitous or erythroid trans...
متن کاملActivity of the rat liver-specific aldolase B promoter is restrained by HNF3.
Although it contains binding sites for HNF1, NFY and C/EBP/DBP, the proximal promoter of the aldolase B gene is surprisingly weak when tested by transient transfection in differentiated hepatoma cells. This low activity could be due to overlapping between HNF1 and HNF3 binding sites in element PAB, from -127 to -103 bp with respect to the cap site. Replacement of the PAB region by a consensus H...
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Mammalian housekeeping promoters represent a class of regulatory elements different from those of tissues-specific genes, lacking a TATA box and associated with CG-rich DNA. We have compared the organization of the housekeeping Htf9 promoter in different cell types by genomic footprinting. The sites of in vivo occupancy clearly reflected local combinations of tissue-specific and ubiquitous bind...
متن کاملA testis-specific promoter in the rat vasopressin gene.
In the rat testis, the vasopressin gene is transcribed into precursor RNAs that are processed into a number of mature transcripts. One of these transcripts has a structure identical to that of the hypothalamic RNA that encodes the vasopressin prepropeptide, but is present at such low levels that it can only be detected by the polymerase chain reaction. Other vasopressin-like RNAs are derived fr...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1989
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1989.tb14690.x